IT is now definitely established that ergothioneine is present in the corpuscles of human blood and of certain animal bloods. Hunter and Eagles [1925] isolated the compound from pig's blood and later [1927] referred to its isolation from children's blood. Benedict, Newton and Behre [1926] also isolated the . substance from both pig's and human blood. Hunter [1928] has briefly sur- veyed the history of ergothioneine and has pointed out the importance of two delicate colour tests to which the substance responds, the development of a blue colour with the uric acid reagents and a new reaction developed by Hunter from the Ehrlich diazo-reaction. Methods for the estimation of ergothioneine based upon the colour reaction with the uric acid reagents were used by Benedict et al. [1926Benedict et al. [ , 1929, 1, 2], 1, 2], and by Rockwood, In the earlier publication of Benedict, the following quantitative data were given:
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H. B. Salt (1931) studied this question.
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