Studies on the Prophylaxis of Measles with Attenuated Living Virus. IV. Inoculation Tests in Children with Chick Embryo Passage Measles Virus in 1960.

Considci'ably Iargc scalc ficltl trials in an isolatecl \. illagc and a grouji of apariiiTcni 110uses were can'iccl out with our egg-passagcd n. easlcs \, Irus.The inhalation 11tethocl was enlploycd Ihrougl, out.Second and later gencraiioiT chorioallantoic passagcd \, Irus causccl no signs of illness, but ITo tiniform antibody response was obtained.FLITthern, ore 11tc r'sult variecl markcdly lionl batclt to batch of virus prepai'allon.01t the contrary, modified measles virus obtaincd by passagc in the choi'ioalIanioic cavity after 26 scrial passages in the amniotic cavity producecl a higll level or measles antibody in nearly all Ihc \, accmaiccl children., howc\.er, \\. as followcd by clinical responscs, that is "vaccination me as-I'S, " as we reported previously.The symptoms or "vaccination measles" \\, ere a considci'ably high fever ancl ^ash, but willT no symptoms of calahrr or Koplik spots, and the s} ntptoms were nTilder than 111OSc of typical me aslcs.D, naniiie, !! of Firo/rig ,, Tile Refear, /I 111J!1/11/'10, Allcrohia!DJ'J, aJ", Of aka Uni"er$10, , Of aka (R, t, I",,//or 1116/1tn!1011,Ab, ember 30,1960)Our previous papa.(Okuno at a/., 1960c) reported on inoculation tests in man and monkeys with chick embryo passaged measles virusThe first generation of chorioallantois passaged virus material represented socalled "vaccination measles " in some or the inoculated children witlT remarkable antibody response On the contrary, Ilo clinical symptoms were o1)served with second or later generatioll virus material, IJeing followed IJy poor antiliody response.TIT Inonkeys, I'DtlT the second chorioallantois passaged and 26th amniotic passaged measles virus preparations elicited remarkable antibody responses without apprecial)Ie clinical manifestations when administered in a large dose by the inhalation or the injection method.Consequently in the present study, larger scale field trials were carried out with chorioallantoic passaoed and Inodined amniotic passaged measles virus preparations.\, Vhen a definite dose of modified amniotic passaged virus was givei} to children by the inhalation method, nearly all children showed symptoms of "vaccination measles, " SU\.I: