Key result
A handheld microfluidic chip based on colorimetric LAMP detected PEDV, TGEV, PoRV, and PCV2 within 60 minutes with a limit of detection of 100 genomic copies, showing excellent concordance with RT-PCR.
Why the study?
Conventional molecular diagnostic methods for porcine diarrhea-related viruses require skilled personnel and sophisticated equipment, restricting their use in centralized laboratories and limiting rapid, on-site multiplex detection.
Does a handheld microfluidic chip accurately detect porcine diarrhea-related viruses compared to standard RT-PCR?
Does a handheld microfluidic chip accurately detect porcine diarrhea-related viruses compared to standard RT-PCR?
A novel handheld microfluidic chip provides rapid, specific, and sensitive on-site multiplex detection of four major porcine diarrhea-related viruses.
May enable faster on-farm detection of swine viruses; leaves open need for field validation before routine use.
The porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PoRV), and porcine circovirus type 2 (PCV2) of neonatal pigs cause great economic losses to pig farms and pork processing enterprises. Specific, rapid, and simple multiplex detection of these viruses is critical to enable prompt implementation of appropriate control measures. Conventional methods for molecular diagnosis require skilled personnel and relatively sophisticated equipment, restricting their use in centralized laboratories. A low-cost, rapid, and field-deployable handheld chip was developed for visual codetection of PEDV, TGEV, PoRV, and PCV2 within 60 min based on colorimetric loop-mediated isothermal amplification (LAMP). The assay’s analytical performance is comparable with that of a real-time RT-LAMP assay and the gold standard quantitative reverse transcription polymerase chain reaction (RT-PCR) assay with limits of detection of 100 genomic copies for all PEDV, TGEV, PoRV, and PCV2. Evaluation of clinical specimens from diseased pigs using our handheld chip revealed excellent concordance with both benchtop LAMP and RT-PCR. The portable handheld LAMP microfluidic chip will potentially facilitate simple, specific, and rapid multiplexed detection of infection disease in minimally equipped veterinary diagnostic laboratories and on-site in pigs’ farms.
No takes yet. Share an insight, caveat, or question.
Wen et al. (2022) studied Porcine diarrhea-related viral infections. Handheld microfluidic chip based on colorimetric LAMP vs. Real-time RT-LAMP and quantitative RT-PCR was evaluated on Limit of detection and concordance with benchtop assays. A handheld microfluidic chip based on colorimetric LAMP detected PEDV, TGEV, PoRV, and PCV2 within 60 minutes with a limit of detection of 100 genomic copies, showing excellent concordance with RT-PCR.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: