Key result
A novel multiplex TaqMan probe-based real-time PCR assay simultaneously detected and differentiated PEDV G1, PEDV G2, RVA, and RVC with 100% concordance to standard methods in 88 swine clinical samples.
Why the study?
Due to high prevalence and frequent co-infection of PEDV genotypes G1 and G2 alongside porcine rotavirus groups A and C, close monitoring is necessary to prevent significant economic losses.
Does a multiplex TaqMan probe-based real-time PCR accurately detect and differentiate PEDV subtypes G1 and G2, RVA, and RVC in swine clinical samples compared to standard methods?
Does a multiplex TaqMan probe-based real-time PCR accurately detect and differentiate PEDV subtypes G1 and G2, RVA, and RVC in swine clinical samples compared to standard methods?
The developed multiplex real-time PCR provides a highly sensitive and accurate method for the rapid simultaneous detection of major porcine diarrheal viruses.
Supports multiplex swine virus detection in research labs; leaves open clinical adoption pending prospective validation.
Porcine viral diarrhea diseases affect the swine industry, resulting in significant economic losses. Porcine epidemic diarrhea virus (PEDV) genotypes G1 and G2, and groups A and C of the porcine rotavirus, are major etiological agents of severe gastroenteritis and profuse diarrhea, particularly among piglets, with mortality rates of up to 100%. Based on the high prevalence rate and frequent co-infection of PEDV, RVA, and RVC, close monitoring is necessary to avoid greater economic losses. We have developed a multiplex TaqMan probe-based real-time PCR for the rapid simultaneous detection and differentiation of PEDV subtypes G1 and G2, RVA, and RVC. This test is highly sensitive, as the detection limits were 20 and 100 copies/μL for the G1 and G2 subtypes of PEDV, respectively, and 50 copies/μL for RVA and RVC, respectively. Eighty-eight swine clinical samples were used to evaluate this new test. The results were 100% in concordance with the standard methods. Since reassortment between porcine and human rotaviruses has been reported, this multiplex test not only provides a basis for the management of swine diarrheal viruses, but also has the potential to impact public health as well.
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Zhang et al. (2022) studied Porcine viral diarrhea (n=88). Multiplex TaqMan probe-based real-time PCR vs. Conventional PCR and sequencing was evaluated on Concordance with standard methods. A novel multiplex TaqMan probe-based real-time PCR assay simultaneously detected and differentiated PEDV G1, PEDV G2, RVA, and RVC with 100% concordance to standard methods in 88 swine clinical samples.
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