Genetic Diversity of Porcine Group A Rotavirus Strains from Pigs in South Korea

Figures

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  Figure 1. Regional prevalence of 53 PoRVA strains detected in Korean pigs between 2014 and 2018.

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  Figure 2. Comparison of the distribution of the G and P combinations of PoRVA strains that have circulated in pigs in South Korea. Distribution of the G/P combinations of PoRVA strains during 2006–2007 (n = 98) [17] (A) and 2014–2018 (n = 53) (B).

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  Figure 3. Phylogenetic analysis based on a partial nucleotide sequence of the VP7 gene from 53 PoRVA antigen-positive samples and selected reference strains. The phylogenetic tree was reconstructed using the maximum-likelihood (ML) method (based on the Tamura 3-parameter model) in the MEGA X program, and tested using 1000 bootstrap values. The 53 Korean PoRVA isolates identified in this study are denoted by blue letters.

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  Figure 4. Phylogenetic analysis based on a partial nucleotide sequence of the VP4 gene from 53 PoRVA antigen-positive samples and selected reference strains. The phylogenetic tree was reconstructed based on the HKY (Hasegawa-Kishino-Yano) model in the MEGA X program, with 1000 bootstrap values. The 53 Korean PoRVA isolates identified in this study are denoted by red letters.

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  Figure 5. Isolation and detection of the PoRVA CN-202 (G4P[13]) and CN-67 (G9P[7]) strains in TF-104 cells. TF-104 cells were seeded in T-75 flasks and infected with 10th passage virus at a multiplicity of infection (MOI) of 0.1. Specific cytopathic effects were observed under a microscope at 24–72 h postinfection (hpi) (Mag × 100). The immunofluorescence assay results for the PoRVA CN-202 (G4P[13]) and CN-67 (G9P[7]) strains (passage 10) in infected TF-104 cells at 20 hpi are shown (Mag × 100).