Key result
Porcine circovirus-like virus was detected in 11.2% of diarrhoea samples and 13.3% of pig farms, suggesting an association with severe porcine diarrheal disease in China.
Why the study?
The prevalence and pathogenicity of porcine circovirus-like virus, a CRESS DNA virus associated with severe disease across diverse hosts, are rarely studied.
Observational (n=178)
Yes
Porcine circovirus-like virus is associated with severe diarrheal disease in pigs in China and shows high homology to bovine circovirus-like virus.
BACKGROUND: The circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus emergence in diverse host has been associated with severe disease. Porcine circovirus-like virus (Po-Circo-like [PCL] virus) is a CRESS DNA virus, the prevalence and pathogenicity of which are rarely studied. METHODS: We obtained two blood samples, four faecal samples, and two intestinal samples from a pig farm suffered from diarrheal disease in the delivery room in September 2020 and attempted to isolate and identify a causative pathogen. Subsequently, only PCL virus was positive, and qRT-PCR was designed to detect the loading titre of PCL virus. We then initiated a heightened surveillance program on the pathogenicity and epidemiology of PCL virus. RESULTS: Six PCL virus strains, with severe diarrhoea and haemorrhagic enteritis, have been found in six different pig farms in Guangdong province, China. A multiple sequence alignment of these PCL viruses and bovine circovirus-like virus/CH showed a similarity of 92.5-94.8% for the Rep protein, indicating these PCL viruses are highly homologous to Bo-Circo-like virus associated with calf diarrhoea. There were striking similarities between the PCL virus and bovine circovirus-like virus outbreaks in aetiological settings and Genomic sequence. We found that 11.2% (20/178) of diarrhoea samples and 13.3% (6/45) of pig farms were positive for PCL virus, suggesting that PCL virus may have spread widely in Pig farms. Moreover, this article underscores the risk of PCL virus spilling over and adapting to new species. CONCLUSIONS: Porcine circovirus-like virus was found to be associated with porcine diarrheal disease in China.
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Liu et al. (2021) conducted an observational in Porcine diarrheal disease (n=178). Porcine circovirus-like virus (PCL virus) infection was evaluated on PCL virus positivity rate in diarrhoea samples. Porcine circovirus-like virus was detected in 11.2% of diarrhoea samples and 13.3% of pig farms, suggesting an association with severe porcine diarrheal disease in China.
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