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The effects of gaseous permeability of the film and storage temperature (0° and 4°C) on the microflora of refrigerated beef were analyzed; total aerobic counts, anaerobic counts, Pseudomonas sp., Brochothrix thermosphacta, Lactobacillus sp., Enterobacteriaceae and yeasts were determined as functions of time. Storage periods to reach a total count of 10 7 CFU/cm 2 were established for different conditions. Microbial growth was modeled by mathematical equations in terms of initial contamination, growth rate and length of the lag phase for each component of the mixed flora.
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Zamora et al. (1985) studied this question.
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