The plasma concentrations of testosterone and luteinizing hormone (LH) were measured by radioimmunoassay during the ovulation cycle of the hen. The baseline concentrations of testosterone were 100 to 250 pg/ml and the maximum concentration of 904 pg/ml was observed 8 h before ovulation. The maximum pre-ovulatory concentration of LH of 2.0 ng/ml, which was approximately twice the basal concentration, was observed 6 h before ovulation. The possibility that testosterone is the ovarian excitation hormone which initiates the ovulation-inducing surge of LH is discussed.
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Etches et al. (1977) studied this question.