The aflatoxins content of 140 cotton seed samples were determined using high-performance liquid chromatography. Samples were obtained from wholesalers in Iran between May 2010 and June 2011. Aflatoxin B₁ gave the highest incidence of contamination and was found in 129 of the 139 samples. The highest concentration of aflatoxin was 14.4 ng g⁻¹. Thirteen cotton seed samples (9.35%) were above one of the regulatory limits of the European Union (5 ng g⁻¹), but no sample was above the highest EU limit and the safety limit recommended by the FDA (20 ng g⁻¹) and regulatory limits of Iran (50 ng g⁻¹) for total aflatoxin.
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Feizy et al. (2012) studied this question.
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