Key result
Translation directed by the HCV IRES, PTV IRES, or EMCV IRES is enhanced by the HCV 3'-UTR or by a poly(A)-tail in different cell types.
The HCV 3'-UTR enhances IRES-dependent translation initiation across different viral IRES elements in various cell types.
Requires validation before any therapeutic consideration; leaves open the in vivo relevance of 3'-UTR enhancement for IRES-driven translation.
Translation of hepatitis C virus (HCV) genomic RNA is directed by an internal ribosome entry site (IRES) in the 5'-untranslated region (5'-UTR), and the HCV 3'-UTR enhances IRES activity. Since the HCV 3'-UTR has a unique structure among 3'-UTRs, we checked possible communication between the 5'- and the 3'-UTR of HCV during translation using chimeric reporter RNAs. We show that translation directed by the HCV IRES and by the HCV-like IRES of porcine teschovirus (PTV) which belongs to a quite distinct family of viruses (picornaviruses) or by the EMCV IRES is also enhanced by the HCV 3'-UTR or by a poly(A)-tail in different cell types.
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Bung et al. (2010) studied Hepatitis C virus (HCV) translation. HCV 3'-UTR or poly(A)-tail was evaluated on Translation enhancement. Translation directed by the HCV IRES, PTV IRES, or EMCV IRES is enhanced by the HCV 3'-UTR or by a poly(A)-tail in different cell types.
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