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Mesophilic Methanothrix sp. strains MTAS and MTKO were isolated as pure cultures from the sludge of anaerobic digestors. We examined the relationships between these two isolates and three other Methanothrix strains (strains OpfikonT [T = type strain], GP6T, and FE) by using an immunological method, by performing DNA homology experiments, and by analyzing polar lipids. Our results indicate that all five strains belong to the same species.
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Ohtsubo et al. (1991) studied this question.
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