It is shown that treatments with nitrous acid, formaldehyde, hypochlorite solutions, and light will insolubilize the silk proteins. Fibroin can also be made insoluble by impregnation with caustic soda followed by heating, but this treatment does not make sericin insoluble. Explanations are advanced, where possible, of the insolubilizing effect of these treatments.
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Cadwallader et al. (1956) studied this question.
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