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Fertilization of the rat ovum occurs before mass displacement of the surrounding follicle cells; denudation of the ova occurs subsequently. Since hyaluronidase, introduced into the uterus, does not pass into the tubes to denude the ova, it seems that only the enzyme associated with the sperm which reach the oviduct disperses the follicle cells.
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Leonard et al. (1947) studied this question.
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