Insulin, yeast hexokinase, or a combination of both substances increased the rate of fructolysis of washed, phosphate buffer-suspended bovine spermatozoa. Seminal plasmas from the same ejaculates suppressed insulin-hexokinase effects. Glucose uptake was not affected by insulin, and hexokinase depressed it. Insulin and hexokinase together, however, increased glucolysis by sperm. When glucose and fructose were in the same media only the former was appreciably utilized and its uptake was increased by the insulin-hexokinase combination.
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Masken et al. (1964) studied this question.
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