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Methanosarcina barkeri strain FR-19 lysed spontaneously in substrate-depleted cultures. The addition of 0.3 m-sucrose prevented complete lysis and resulted in the formation of osmotically sensitive UV-fluorescent spheres. Electron microscopic examination showed that complete degradation of the cell wall occurred before the release of true protoplasts, which were stabilized by sucrose and glucose (0·2–0·3 m) but not by glycerol. Exponentially increasing methane production and regeneration of protoplasts could not be demonstrated.
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Davis et al. (1985) studied this question.
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