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May 1, 2002Antimicrobial Agents and ChemotherapyOpen Access

High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

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Authors

IMIgor Mokrousov ONOlga Narvskaya TOTatiana Otten

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Overview

Surveillance study reveals a high prevalence of the katG S315T mutation in drug-resistant tuberculosis isolates, indicating a target for rapid molecular testing.

Key Points

Determine the prevalence of the katG Ser315Thr mutation among isoniazid-resistant Mycobacterium tuberculosis clinical isolates and evaluate a PCR-RFLP assay for rapid detection.
Screened 204 isoniazid-resistant and 57 isoniazid-susceptible M. tuberculosis clinical isolates collected from northwestern Russia (1996–2001) using a HapII-based PCR-RFLP assay.
Genotyped resistant isolates using IS6110-RFLP analysis and spoligotyping to identify Beijing family strains and assessed test specificity against culture-based drug susceptibility testing.
The katG S315T mutation was detected in 93.6% of isoniazid-resistant isolates and in 0% of 57 susceptible isolates, yielding a diagnostic specificity of 100%.
The Beijing genotype accounted for 60.3% of resistant strains and exhibited a higher frequency of the S315T substitution than non-Beijing strains (97.8% vs. 84.6% in 1999–2001 isolates; 100.0% vs. 86.5% among new cases from 1996–2001).

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Mokrousov et al. (2002) studied this question.

synapsesocial.com/papers/6a9464d64efb9d310ac7d0ae https://doi.org/10.1128/aac.46.5.1417-1424.2002

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May 1, 2002Antimicrobial Agents and ChemotherapyOpen Access

High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

View Full Paper

Ask AI

Bookmark

Authors

IMIgor Mokrousov ONOlga Narvskaya TOTatiana Otten

Discussion

Member takes

Overview

Surveillance study reveals a high prevalence of the katG S315T mutation in drug-resistant tuberculosis isolates, indicating a target for rapid molecular testing.

Key Points

Determine the prevalence of the katG Ser315Thr mutation among isoniazid-resistant Mycobacterium tuberculosis clinical isolates and evaluate a PCR-RFLP assay for rapid detection.
Screened 204 isoniazid-resistant and 57 isoniazid-susceptible M. tuberculosis clinical isolates collected from northwestern Russia (1996–2001) using a HapII-based PCR-RFLP assay.
Genotyped resistant isolates using IS6110-RFLP analysis and spoligotyping to identify Beijing family strains and assessed test specificity against culture-based drug susceptibility testing.
The katG S315T mutation was detected in 93.6% of isoniazid-resistant isolates and in 0% of 57 susceptible isolates, yielding a diagnostic specificity of 100%.
The Beijing genotype accounted for 60.3% of resistant strains and exhibited a higher frequency of the S315T substitution than non-Beijing strains (97.8% vs. 84.6% in 1999–2001 isolates; 100.0% vs. 86.5% among new cases from 1996–2001).

Cite This Study

Mokrousov et al. (2002) studied this question.

synapsesocial.com/papers/6a9464d64efb9d310ac7d0ae https://doi.org/10.1128/aac.46.5.1417-1424.2002

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