Key result
Unlike Influenza A virus, the putative cap-snatching domains of Thogotovirus PA and PB2 lack conserved active site residues and do not exhibit endonuclease or cap-binding activities in vitro.
Population
Recombinant Thogotovirus and Dhori virus polymerase domains expressed in E. coli
Comparison
Structural and biochemical analysis vs Influenza A virus (IAV) polymerase domains
Design
Preclinical
Authors
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Cap-snatching inhibitors effective against influenza may lack activity against Thogotovirus; leaves open distinct replication strategies requiring targeted research.
Thogotoviruses possess polymerase domains structurally similar to Influenza A but lack the classical cap-snatching endonuclease and cap-binding activities in vitro.
Guilligay et al. (2014) studied Orthomyxovirus infection (Basic Science). Structural and biochemical analysis of THOV and DHOV polymerase domains vs. Influenza A virus (IAV) polymerase domains was evaluated on Crystal structure determination and in vitro endonuclease/cap-binding activity. Unlike Influenza A virus, the putative cap-snatching domains of Thogotovirus PA and PB2 lack conserved active site residues and do not exhibit endonuclease or cap-binding activities in vitro.
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