Key result
Chemical mapping revealed a Mg(2+)-dependent pseudoknot structure containing the PB1-F2 and N40 start codons, suggesting ribosome interactions may affect their translation levels.
The identification of a Mg(2+)-dependent pseudoknot structure in Influenza A segment 2 suggests a mechanism by which the ribosome interacts with the RNA to regulate translation of alternate open reading frames.
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Does not change clinical practice; leaves open ribosome-mediated regulation of influenza alternate ORFs for further study.
Influenza A is a negative-sense RNA virus with an eight-segment genome. Some segments encode more than one polypeptide product, but how the virus accesses alternate internal open reading frames (ORFs) is not completely understood. In segment 2, ribosomal scanning produces two internal ORFs, PB1-F2 and N40. Here, chemical mapping reveals a Mg(2+)-dependent pseudoknot structure that includes the PB1-F2 and N40 start codons. The results suggest that interactions of the ribosome with the pseudoknot may affect the level of translation for PB1-F2 and N40.
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Priore et al. (2015) studied Influenza A. Chemical mapping of RNA structure was evaluated on Identification of RNA pseudoknot structure. Chemical mapping revealed a Mg(2+)-dependent pseudoknot structure containing the PB1-F2 and N40 start codons, suggesting ribosome interactions may affect their translation levels.
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