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A low-ionic strength-Polybrene (LISP) AutoAnalyzer haemagglutination channel (Lalezari, 1968; Perrault and Hogman, 1971) fails to detect a proportion of antibodies, particularly anti-KI, anti-Lewis, and anti-P1, which can be demonstrated by manual techniques (Moore and Perrrault, 1977). Moore et al. (1977) found that when dialysis was used instead of dilution to achieve a low ionic-strength reaction environment (dialysed serum-Polybrene or DISP), a greater proportion of anti-KI and anti-Lewis antibodies was detected.
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Crockford et al. (1979) studied this question.
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