Key result
Purification of oral poliovaccine suspensions using Q-Sepharose Fast Flow chromatography achieved 100% recovery of virus infectivity with residual substrate DNA less than 100 pg/dose.
Q-Sepharose Fast Flow chromatography is an effective method for purifying Vero cell-derived oral poliovaccine, achieving high virus recovery and low residual DNA without altering biological characteristics.
Q-Sepharose Fast Flow (Q-Sepharose F. F.) was adopted to purify the suspensions of type I, II, III oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Using column chromatography on Q-Sepharose F. F. to purify the concentrated virus suspensions, the purified viruses attain 100% recovery of virus infectivity. Dot membrane hybrization was used to detect DNA with the probe of Vero cell genome DNA which was labeled with α-P 32 dATP, the contents of residual substrate DNA was less than 100 Pg/dose. The process of downstream had no significant influence on some biological characters of purified viruses, such as virus morphology, tumorigenicity, rct/40 character and capsid protein. This downstream reseach indicates that Q-Sepharose F. F. is an ideal chromatography material for purifying OPV prepared from Vero cells.
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GuoYang et al. (1999) studied Oral poliovaccine (OPV) purification. Q-Sepharose Fast Flow chromatography was evaluated on Recovery of virus infectivity and residual substrate DNA content. Purification of oral poliovaccine suspensions using Q-Sepharose Fast Flow chromatography achieved 100% recovery of virus infectivity with residual substrate DNA less than 100 pg/dose.
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