Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode

Down to the bare bones: When a glucose oxidase (GOx, left) was deglycosylated, the modified enzyme (right) immobilized on a vitreous carbon electrode came into closer electrical contact with the surface than the native enzyme. Glucose was electrooxidized directly on a monolayer of deglycosylated GOx on a carbon electrode at the unprecedented reducing potential of −200 mV versus Ag/AgCl and with a current density of 235 μA cm−2.