A simple HPLC procedure for the identification and quantitation of phytochelatins (PCs) in plant tissue extracts is described. The method, which does not require a derivatization step utilizes only 20 µL of sample volume. Linear quantitative response curve was generated for phytochelatin 3 (PC3) over a concentration range of 1.33 µmol/L–6.66 mmol/L. Linear regression analysis of the standard curve exhibited correlation coefficient of 0.996. Limit of detection (LOD) and limit of quantitation (LOQ) values were 0.1 and 0.5 µmol, respectively. Phytochelatin 3 recovery using this method was relatively high (above 85%). Intra‐assay and inter‐assay precision studies reflected a high level of reliability and reproducibility of the method. The applicability of the method for the quantitation of a wide range of PC concentrations in plant tissue extracts was demonstrated successfully.
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Hunaiti et al. (2003) studied this question.
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