Media for Selective Isolation of Hymenomycetes

The growth rates of 32 hymenomycetes and non-hymenomycetes were compared on five media that have been devised for isolation of hymenomycetes. A medium with 2 mg/L benomyl permitted virtually uninhibited growth of hymenomycetes while strongly inhibiting many non-hymenomycetes, but members of the Mucorales and several other species still grew well. Testing of various combinations and concentrations of ingredients led to the use of 2 mg/L benomyl and 2 mg/L dichloran as antifungal ingredients. This was tested in isolations from various field materials and in every case performed as well as or better than other commonly used media.