What question did this study set out to answer?

To identify binding partners of phosphodiesterase 3B (PDE3B) in adipocytes and determine the molecular mechanism regulating its activation by insulin.

December 1, 2002Diabetes

Identification of the Insulin-Regulated Interaction of Phosphodiesterase 3B With 14-3-3 β Protein

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Authors

HOHiroshi OnumaEhime University HOHaruhiko OsawaHiroshima University KYKazuya YamadaSaitama University

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Implication

Biochemical study reveals that insulin enhances phosphodiesterase 3B binding to 14-3-3 beta in rat adipocytes via PI3-kinase, suggesting a scaffolding role in antilipolytic signaling.

Key Points

To identify binding partners of phosphodiesterase 3B (PDE3B) in adipocytes and determine the molecular mechanism regulating its activation by insulin.
Screened for PDE3B-interacting proteins using a yeast two-hybrid system and validated bindings in vitro with GST-tagged 14-3-3 beta.
Conducted co-immunoprecipitation in rat adipocytes following insulin treatment and PI3-kinase inhibition with wortmannin and LY294002.
Tested competitive binding using synthetic phosphorylated peptides targeting rat PDE3B serine residues 279 and 302.
Insulin treatment markedly enhanced the association between endogenous PDE3B and endogenous 14-3-3 beta in rat adipocytes.
Inhibition of PI3-kinase with wortmannin or LY294002 completely blocked the insulin-induced interaction between PDE3B and 14-3-3 beta.
Synthetic PDE3B peptides phosphorylated at Ser-279 or Ser-302 inhibited complex formation, demonstrating that phosphorylation of these serine sites mediates binding.

Cite This Study

Onuma et al. (2002) studied this question.

synapsesocial.com/papers/6a94d04d5f3b0bfa3be60e75 https://doi.org/10.2337/diabetes.51.12.3362

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Implication

Biochemical study reveals that insulin enhances phosphodiesterase 3B binding to 14-3-3 beta in rat adipocytes via PI3-kinase, suggesting a scaffolding role in antilipolytic signaling.

Identification of the Insulin-Regulated Interaction of Phosphodiesterase 3B With 14-3-3 β Protein

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of the Insulin-Regulated Interaction of Phosphodiesterase 3B With 14-3-3 β Protein

Discussion

Member takes

Implication

Key Points

Cite This Study

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