Use of Integrated Rate Equations in Estimating the Kinetic Constants of Enzyme-catalyzed Reactions

Methods are described for estimation of kinetic constants from the integrated rate equations for a number of reaction mechanisms. In general, these methods depend upon elimination of either zero order or first order terms between rate curves determined at two initial substrate concentrations or more. This operation is followed by graphic or statistical evaluation of the kinetic constants in the simplified equation and substitution of these values back into the original rate equation to permit evaluation of the remaining kinetic constants.