Effects of irbesartan on myocardial injury in diabetic rats: The role of NLRP3/ASC/Caspase-1 pathway

Figures

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  Figure 1.. The changes of triglyceride (TG), total cholesterol (TC) and fasting blood glucose (FBG) levels in different groups (mean±SD, n=6). The plasma (a) TG, (b) TC and (c) FBG levels in different groups. **p<0.01 vs. CON ^^p<0.01 vs. HC. CON: control group; HC: high glucose and high fat group; DM: diabetes group; DM+Ir: diabetes+irbesartan group.

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  Figure 2.. The heart functions in the different groups (mean±SD, n=6). The (a) IVS(d), (b) LVID(d), (c) LVPW(d) and EF% in different groups. (e) Typical echocardiographic images in the different groups. **p<0.01 vs. CON ^^p<0.01 vs. HC ##p<0.01 vs. DM. CON: control group; HC: high glucose and high fat group; DM: diabetes group; DM+Ir: diabetes+irbesartan group; IVS(d): interventricular septum diameter; LVID(d): left ventricular internal diameter at end-diastole; LVPW(d): left ventricular posterior wall diameter; EF%: ejection fraction percentage.

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  Figure 3.. Masson staining of rat myocardial tissues in different groups (original magnification: 100×, n=4 ). (a) Control group; (b) high glucose and high fat group; (c) diabetes group; (d) diabetes+irbesartan group.

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  Figure 4.. Changes of NLRP3, ASC and Caspase-1 levels in the myocardial tissues (mean±SD, n=6). (a) NLRP3, (b) ASC and (c) Caspase-1 mRNA levels in myocardial tissues. **p<0.01 vs. CON ^^p<0.01 vs. HC ##p<0.01 vs. DM. NLRP3: nucleotide binding oligomerization domain-like receptor protein 3; ASC: apoptosis-associated speck-like protein containing CARD; Caspase-1: cysteinyl aspartate specific proteinase-1; CON: control group; HC: high glucose and high fat group; DM: diabetes group; DM+Ir: diabetes +irbesartan group.

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  Figure 5.. Changes of NLRP3, ASC and Caspase-1 at protein expressions in different groups (mean+SD, n=6). (a) Typical Western blot bands of NLRP3, ASC and Caspase-1 protein expressions; B, C, D: Statistical analysis of (b) NLRP3, (c) ASC and (d) Caspase-1 protein expression. **p<0.01 vs. CON ^^p<0.01 vs. HC ##p<0.01 vs. DM. NLRP3: nucleotide binding oligomerization domain-like receptor protein 3; ASC: apoptosis-associated speck-like protein containing CARD; Caspase-1: cysteinyl aspartate specific proteinase-1; CON: control group; HC: high glucose and high fat group; DM: diabetes group; DM+Ir: diabetes +irbesartan group.

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  Figure 6.. Enzyme immunoassay for detecting the levels of interleukin (IL)-18 and IL-1β in myocardial tissue (mean±SD, n=6). (a) The IL-1β level in different groups; (b) the IL-18 level in different groups. **p<0.01 vs. CON ^^p<0.01 vs. HC ##p<0.01 vs. DM CON: control group; HC: high glucose and high fat group; DM: diabetes group; DM+Ir: diabetes+ irbesartan group.