A Comparative Study of the Structures of Xyloglucans from the Fruit Cell Walls of Four Apple Cultivars.

The oligosaccharide units of xyloglucans from four apple cultivars were analyzed by enzymatic digestion followed by anion-exchange chromatography with pulsed amperometric detection. The oligosaccharide units of the polysaccharides were XXXG, XXLG, XLXG, XXFG, XLLG and XLFG [where each (1→4)-β-linked D-glucosyl residue in the backbone is given a one-letter code according to its substituents: G=β-D-Glc;X=α-D-Xyl-(1→6)-β-D-Glc;L=β-D-Gal-(1→2)-α-DXyl-(1→6)-β-D-Glc;F=α-L-Fuc-(1→2)-(3a)-Gal-(1→2)-a-D-Xyl-(1→6)-β-D- Glc] in an apProx mate molar ratio of 23 :9: 12:26: 14: 16 for 'Starking Delicious, ' of 26:7 :7:28: 8 :24 for `Fuji, ' of 24:3 :8:27:5 :33 for `Ohrin, ' and of 24:2:8:28 : 3 :35 for `Ralls Janet (Kokko).' Present result clearly shows that apple xyloglucan contains structural oligosaccharide units, XXLG, XLXG and XLLG in addition to XXXG, XXFG and XLFG.