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September 1, 1996AJP Endocrinology and Metabolism

IGFBP-3 proteolysis by plasmin, thrombin, serum: heparin binding, IGF binding, and structure of fragments

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Key result

Exposure of IGFBP-3 to plasmin, thrombin, and serum generated multiple proteolytic fragments with differential affinities for IGF and heparin.

Population

Insulin-like growth factor binding protein (IGFBP)-3 (in vitro model)

Design

Preclinical

Authors

BBBarbara A. BoothUniversity of IowaMBM. BoesChildren's MinnesotaRBRobert S. BarUniversity of Iowa

Discussion

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Implication

May influence vascular IGF bioavailability experimentally; leaves open effects on endothelial function in vivo.

Structured PICO

P
Population
Insulin-like growth factor binding protein (IGFBP)-3 (in vitro model)
E
Exposure
Exposure to plasmin, thrombin, and pregnancy serum
O
Outcome
Generation of proteolytic fragments, their NH2-termini sequences, and binding affinity for IGF and heparinsurrogate

Proteolysis of IGFBP-3 by endothelial surface substances generates fragments with varying affinities for IGF and heparin, which may influence vascular IGF concentrations and endothelial function.

Cite This Study

Booth et al. (1996) studied this question. Plasmin, thrombin, and pregnancy serum was evaluated on Proteolytic fragmentation of IGFBP-3 and binding ability to IGF and heparin. Exposure of IGFBP-3 to plasmin, thrombin, and serum generated multiple proteolytic fragments with differential affinities for IGF and heparin.

synapsesocial.com/papers/6a94d7a557dad826d8f579edhttps://doi.org/10.1152/ajpendo.1996.271.3.e465
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Insulin-Like Growth Factor Binding Proteins in Maternal Serum Throughout Gestation and in the Puerperium: Effects of a Pregnancy-Associated Serum Protease Activity*1990 · 367 citations
  2. 2Prostate-specific antigen (PSA) is an insulin-like growth factor binding protein-3 protease found in seminal plasma.1992 · 478 citations
  3. 3Endothelial cells express insulin-like growth factor-binding proteins 2 to 6.1992 · 62 citations
  4. 4A major portion of the 150-kilodalton insulin-like growth factor-binding protein (IGFBP) complex in adult rat serum contains unoccupied, proteolytically nicked IGFBP-3 that binds IGF-II preferentially.1995 · 36 citations
  5. 5Identification and partial characterization of a proteinase specific for insulin-like growth factor binding protein-3 in aqueous and vitreous humors1995 · 11 citations