What question did this study set out to answer?

To evaluate the effect of retinoic acid and retinol on neurite outgrowth in the developing amphibian spinal cord and map their endogenous distribution.

May 1, 1991Proceedings of the National Academy of SciencesOpen Access

Retinoic acid stimulates neurite outgrowth in the amphibian spinal cord.

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To evaluate the effect of retinoic acid and retinol on neurite outgrowth in the developing amphibian spinal cord and map their endogenous distribution.
Cultured explanted segments of axolotl spinal cord treated with varying concentrations of retinoic acid or retinol.
Quantified endogenous tissue levels of retinoic acid and retinol using high-performance liquid chromatography (HPLC).
Mapped cellular retinoic acid-binding protein (CRABP) and cellular retinol-binding protein (CRBP) distribution via immunocytochemistry with antipeptide antibodies.
Retinoic acid stimulated neurite outgrowth by 4- to 5-fold above a threshold concentration of 0.1 to 1 nM, whereas retinol stimulated only glial cell migration.
HPLC confirmed endogenous presence of both retinoic acid and retinol within axolotl spinal cord tissue.
Immunocytochemical analysis identified CRABP selectively in axons, whereas CRBP localized to ependymal and glial cells.

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Implication

Ex vivo study demonstrates that retinoic acid induces neurite outgrowth in axolotl spinal cord explants, highlighting a key role in nervous system development.

Cite This Study

Hunter et al. (1991) studied this question.

synapsesocial.com/papers/6a94ea5c57dad826d8f57f57 https://doi.org/10.1073/pnas.88.9.3666

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Implication

Ex vivo study demonstrates that retinoic acid induces neurite outgrowth in axolotl spinal cord explants, highlighting a key role in nervous system development.

Retinoic acid stimulates neurite outgrowth in the amphibian spinal cord.

Key Points

Discussion

Implication

Cite This Study

Retinoic acid stimulates neurite outgrowth in the amphibian spinal cord.

Key Points

Discussion

Implication

Cite This Study

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