Key result
Obesity was associated with higher VDR mRNA levels (VAT: P=0.04, SAT: P<0.0001) and lower CYP27B1 levels (VAT: P=0.004, SAT: P=0.016) in adipose tissue compared to normal-weight controls.
Why the study?
It was unknown whether obesity alters vitamin D-related gene expression in adipose tissue, whether microRNA interference plays a role, and how this affects the local inflammatory milieu.
Cross-Sectional (n=86)
Obesity is associated with up-regulation of VDR and down-regulation of CYP27B mRNA levels in adipose tissue, which correlates with pro-inflammatory cytokine expression and may be regulated by miRNAs.
Altered adipose VDR expression in obesity correlates with inflammation; leaves open miRNA regulation and any clinical implications.
Background: Given the role that vitamin D (VD) plays in the regulation of the inflammatory activity of adipocytes, we aimed to assess whether obesity changes the expression of VD-related genes in adipose tissue and, if so, to investigate whether this phenomenon depends on microRNA interference and how it may influence the local inflammatory milieu. Methods: The expression of genes encoding VD 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1) and receptor (VDR), selected interleukins and microRNAs was evaluated by real-time PCR in visceral (VAT) and in subcutaneous (SAT) adipose tissues of 55 obese (BMI > 40 kg/m2) and 31 normal-weight (BMI 20–24.9 kg/m2) individuals. Results: VDR mRNA levels were higher, while CYP27B1 levels were lower in adipose tissues of obese patients than in those of normal-weight controls (VAT: P = 0.04, SAT: P < 0.0001 and VAT: P = 0.004, SAT: P = 0.016, respectively). The expression of VDR in VAT of obese subjects correlated negatively with levels of miR-125a-5p (P = 0.0006, rs = −0.525), miR-125b-5p (P = 0.001, rs = −0.495), and miR-214-3p (P = 0.009, rs = −0.379). Additionally, VDR mRNA concentrations in visceral adipose tissues of obese subjects correlated positively with mRNA levels of interleukins: 1β, 6 and 8. Conclusions: We observed obesity-associated up-regulation of VDR and down-regulation of CYP27B mRNA levels in adipose tissue. VDR expression correlates with the expression of pro-inflammatory cytokines and may be regulated by miRNAs.
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Jonas et al. (2019) conducted a cross-sectional in Obesity (n=86). Obesity (BMI > 40 kg/m2) vs. Normal-weight (BMI 20-24.9 kg/m2) was evaluated on VDR and CYP27B1 mRNA levels in visceral and subcutaneous adipose tissues. Obesity was associated with higher VDR mRNA levels (VAT: P=0.04, SAT: P<0.0001) and lower CYP27B1 levels (VAT: P=0.004, SAT: P=0.016) in adipose tissue compared to normal-weight controls.
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