To the Editor. —Biochemical characteristics of the vitreous have been studied in animals, but very few studies have been conducted in clinical cases.1,2The major difficulty has been the problem of collecting undiluted vitreous samples from human eyes. We have invented a safe method and device for this purpose. Materials and Methods. —A collection bottle with openings on both sides was tightly secured to the suction tubes; one tube, 10 cm in length, was connected to the cutter and the other was fitted to the pump of the vitreous machine. Initally, the vitreous cavity was observed and cut without suction pressure to determine a safe depth and position for the tip of the cutter. Next, the infusion cannula for air was opened and inflated. After collecting a certain amount of vitreous, we stopped the machine, and the air in the infusion pipe was changed to an irrigating solution. We
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Makoto Tamai (1991) studied this question.