Key result
At high magnesium concentrations (5 mM MgCl2), the hepatitis C virus IRES can initiate translation by an alternative mechanism that does not require known initiation factors.
Population
Reconstituted system consisting of purified 40S and 60S subunits, elongation factors, and aminoacylated tRNAs
Comparison
High magnesium concentration (5 mM MgCl2) vs Physiological magnesium ion concentrations
Design
Preclinical
Authors
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No direct clinical implications for HCV; leaves open magnesium's role in IRES translation in physiological models.
The HCV IRES can initiate translation without known initiation factors at high magnesium concentrations, similar to Dicistroviridae IRES elements.
Lancaster et al. (2006) studied Hepatitis C virus translation. High magnesium concentration (5 mM MgCl2) vs. Physiological magnesium ion concentrations was evaluated on Translation initiation mechanism. At high magnesium concentrations (5 mM MgCl2), the hepatitis C virus IRES can initiate translation by an alternative mechanism that does not require known initiation factors.
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