Thermograms of an aerobic batch culture of Escherichia coli K‐12 in synthetic medium were obtained by using a newly designed mecrocalorimeter. The thermograms reflected sharp changes of metabolic activity in glucose‐, nitrogen‐, or oxygen‐limited cultures. The thermo chemical analysis for each culture condition was done using the data of the heat evolved, the head of combustion of cells, and the elementary analysis of the cells. The efficiency of energy conversion determined experimentally revealed nutritional differences.
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Ishikawa et al. (1981) studied this question.
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