Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
April 10, 1999Human Gene Therapy

Expression of the Primary Coxsackie and Adenovirus Receptor Is Downregulated during Skeletal Muscle Maturation and Limits the Efficacy of Adenovirus-Mediated Gene Delivery to Muscle Cells

View Full Paper
Ask AI
Bookmark
Share

Population

Developing mouse muscle from postnatal day 3 to day 60, C2C12 mouse myoblast cells, and dystrophic mdx mice

Comparison

Ectopic expression of coxsackie and adenovirus… vs Control cells or tissues without ectopic CAR…

Design

Preclinical

Authors

JNJoséphine NalbantogluMontreal Neurological Institute and HospitalGPGiovanna PariQueen's UniversityGKGeorge KarpatiMontreal Neurological Institute and Hospital

Discussion

Loading...

Member takes

Implication

Supports CAR overexpression strategies for muscle gene delivery; leaves open translation to in vivo models and clinical use.

Structured PICO

P
Population
Developing mouse muscle (quadriceps, gastrocnemius, heart) from postnatal day 3 to day 60, C2C12 mouse myoblast cells, and dystrophic mdx mice
I
Intervention
Ectopic expression of coxsackie and adenovirus receptor (CAR) followed by adenovirus infection (AVCMVlacZ)
C
Comparator
Control cells or tissues without ectopic CAR expression
O
Outcome
Adenovirus transducibility measured by lacZ reporter gene activitysurrogate

Forced expression of the coxsackie and adenovirus receptor (CAR) in mature skeletal myofibers dramatically increases their susceptibility to adenovirus transduction, overcoming the natural downregulation of CAR during muscle maturation.

Cite This Study

Nalbantoglu et al. (1999) studied this question.

synapsesocial.com/papers/6a950a87fde25ad2081691achttps://doi.org/10.1089/10430349950018409
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Dystrophin Expression in Muscles of mdx Mice After Adenovirus-Mediated <i>In Vivo</i> Gene Transfer1996 · 150 citations
  2. 2Adenoviral-mediated gene transfer in lymphocytes1998 · 127 citations
  3. 3Gene therapy and molecular approaches to the treatment of hereditary muscular disorders2000 · 24 citations
  4. 4Stepwise dismantling of adenovirus 2 during entry into cells1993 · 819 citations
  5. 5Virus-receptor interaction in the adenovirus system: characterization of the positive cooperative binding of virions on HeLa cells1985 · 63 citations