There can be little doubt that the present extensive and fruitful use of chromatographic methods for the separation of water-soluble substances has been stimulated, to a very large degree, by the pioneer investigations of Martin and Synge and their co-workers. They developed the silica gel column for acetyl amino acids, the now famous paper chromatograms, and introduced the use of starch columns (Elsden and Synge, 1944; Synge, 1944). Our own work with chromatographic methods, like that of many others, has been a logical outgrowth of these earlier investigations.
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Stein et al. (1950) studied this question.