What question did this study set out to answer?

To characterize the glycoforms of the secreted purple acid phosphatase AtPAP26 and identify co-purifying regulatory proteins in phosphate-starved Arabidopsis.

August 30, 2018Plant Cell & EnvironmentOpen Access

A glycoform of the secreted purple acid phosphatase AtPAP26 co‐purifies with a mannose‐binding lectin ( AtGAL1 ) upregulated by phosphate‐starved Arabidopsis

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Authors

MGMina GhahremaniInstitute for Advanced Studies in Basic Sciences HTHue TranHanoi Medical University SBSanaz G. BiglouUniversity of Ottawa

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Implication

Biochemical analysis uncovers association of phosphatase AtPAP26-S2 with lectin AtGAL1 in phosphate-starved Arabidopsis, suggesting lectin-mediated enhancement of nutrient scavenging.

Key Points

To characterize the glycoforms of the secreted purple acid phosphatase AtPAP26 and identify co-purifying regulatory proteins in phosphate-starved Arabidopsis.
Purified and analyzed AtPAP26 secreted glycoforms (AtPAP26-S1 and AtPAP26-S2) from phosphate-starved Arabidopsis suspension cells using mass spectrometry.
Identified and characterized the co-purified lectin AtGAL1 via mass spectrometry, thiol-reducing immunoblotting, and expression profiling under phosphate deficiency.
Assessed growth phenotypes of an atgal1 knockout mutant under phosphate-deficient conditions.
Mass spectrometry revealed that AtPAP26-S2 carries modified high-mannose N-glycans at Asn365 and Asn422 and specifically co-purifies with the lectin AtGAL1.
Identified AtGAL1 as a bisphosphorylated, glycosylated 55-kDa lectin with three predicted disulfide bonds in its apple domain.
Secreted AtGAL1 protein levels increased substantially during phosphate starvation despite minimal changes in transcript levels, while atgal1 mutants exhibited no growth defects.

Cite This Study

Ghahremani et al. (2018) studied this question.

synapsesocial.com/papers/6a951bf5229856c3dd1691af https://doi.org/10.1111/pce.13432

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Implication

Biochemical analysis uncovers association of phosphatase AtPAP26-S2 with lectin AtGAL1 in phosphate-starved Arabidopsis, suggesting lectin-mediated enhancement of nutrient scavenging.

A glycoform of the secreted purple acid phosphatase AtPAP26 co‐purifies with a mannose‐binding lectin ( AtGAL1 ) upregulated by phosphate‐starved Arabidopsis

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Implication

Key Points

Cite This Study

A glycoform of the secreted purple acid phosphatase AtPAP26 co‐purifies with a mannose‐binding lectin ( AtGAL1 ) upregulated by phosphate‐starved Arabidopsis

Discussion

Member takes

Implication

Key Points

Cite This Study

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