What question did this study set out to answer?

To demonstrate the feasibility of recording protein powder diffraction patterns from hydrated submicrometer membrane protein nanocrystals using a continuous microjet delivery system.

October 2, 2008Journal of Synchrotron Radiation

Powder diffraction from a continuous microjet of submicrometer protein crystals

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Authors

DSDavid A. ShapiroLawrence Berkeley National Laboratory HCHenry N. ChapmanUniversität Hamburg DDDaniel P. DePonteUniversity of Nebraska–Lincoln

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Implication

Experimental study demonstrates powder diffraction from submicrometer photosystem I crystals using a microjet, highlighting a radiation-free delivery method for serial crystallography.

Key Points

To demonstrate the feasibility of recording protein powder diffraction patterns from hydrated submicrometer membrane protein nanocrystals using a continuous microjet delivery system.
Commissioned a flow-focusing aerojet system designed to stream hydrated nanocrystal solutions directly across an X-ray beam without mechanical crushing or cryocooling.
Introduced suspensions of photosystem I membrane protein nanocrystals with crystallite dimensions under 500 nm into the continuous injector.
Obtained the first room-temperature powder diffraction patterns from photosystem I nanocrystals delivered in a continuous liquid stream.
Observed lowest-order diffraction reflections that showed quantitative agreement with theoretical powder profile calculations.

Cite This Study

Shapiro et al. (2008) studied this question.

synapsesocial.com/papers/6a95283a6738ebe75a330ea4 https://doi.org/10.1107/s0909049508024151

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Implication

Experimental study demonstrates powder diffraction from submicrometer photosystem I crystals using a microjet, highlighting a radiation-free delivery method for serial crystallography.

Powder diffraction from a continuous microjet of submicrometer protein crystals

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Implication

Key Points

Cite This Study

Powder diffraction from a continuous microjet of submicrometer protein crystals

Discussion

Member takes

Implication

Key Points

Cite This Study

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