The aim of the present study was to find the best way of measuring the viability of root and leaf samples from various plant species (pea, wheat and maize) exposed to different concentrations of the heavy metal Cd. A comparison was made of three viability tests, namely electrolyte leakage measurements, and TTC and NBT reduction. The results suggested that electrolyte leakage was the most useful method for measuring leaf viability, being simple, fast, reliable and reproducible. The TTC reduction measurement proved the most useful for maize roots, while NBT reduction was the best method for detecting the viability of pea and wheat roots.
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Gondor et al. (2013) studied this question.
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