Separation and quantitative determination of grape and wine procyanidins by high performance reversed phase liquid chromatography

A method is described for the separation and quantitative analysis of procyanidins in grape and wine. On a polyamide chromatographic column an initial elution with water at pH 7.0 enabled elimination of interfering phenolic acids. A second elution with acetonitrile/water (30:70 v) was performed to extract catechins. A third elution with acetone/water (75:25 v) was carried out to obtain procyanidins. The quantitative determination of procyanidins was done by high performance reversed phase liquid chromatography. The HPLC analysis was brought down to ∼55 min. The method is accurate and reproducible and has an acceptable extraction efficiency.