What question did this study set out to answer?

To investigate the role and molecular mechanisms of microRNA-155 in regulating blood–brain barrier integrity during neuroinflammatory disorders.

March 6, 2014The FASEB Journal

MicroRNA‐155 negatively affects blood–brain barrier function during neuroinflammation

View Full Paper

Ask AI

Bookmark

Authors

MLMiguel Alejandro Lopez‐RamirezUniversity of California, San Diego DWDongsheng WuFirst Affiliated Hospital of Hunan University of Traditional Chinese Medicine GPGareth PryceQueen Mary University of London

Discussion

Member takes

Implication

Preclinical study demonstrates blood-brain barrier breakdown by microRNA-155 in neuroinflammatory models, highlighting its potential as a therapeutic target.

Key Points

To investigate the role and molecular mechanisms of microRNA-155 in regulating blood–brain barrier integrity during neuroinflammatory disorders.
Examined miR-155 expression in brain tissue from individuals with multiple sclerosis and mice with experimental autoimmune encephalomyelitis (EAE).
Measured blood–brain barrier permeability to systemic tracers in miR-155 knockout mice subjected to EAE and lipopolysaccharide-induced acute inflammation.
Assessed junctional integrity, tracer leakage, and target protein expression in cultured human brain endothelial cells following miR-155 overexpression or inhibition.
MicroRNA-155 expression is elevated in human multiple sclerosis and mouse EAE neurovascular units, and genetic deletion of miR-155 significantly decreases tracer extravasation into the central nervous system in mice.
Inflammatory cytokines rapidly induce miR-155 in cultured human brain endothelium, where its overexpression increases barrier permeability, while miR-155 inhibition partially rescues cytokine-induced barrier breakdown.
Mechanistic assays show miR-155 impairs endothelial barrier function by downregulating cell–cell junction components (claudin-1 and annexin-2) and focal adhesion regulators (DOCK-1 and syntenin-1).

Cite This Study

Lopez‐Ramirez et al. (2014) studied this question.

synapsesocial.com/papers/6a9539b18ce716a7e9fa6907 https://doi.org/10.1096/fj.13-248880

View Full Paper

Ask AI

Bookmark

Implication

Preclinical study demonstrates blood-brain barrier breakdown by microRNA-155 in neuroinflammatory models, highlighting its potential as a therapeutic target.

MicroRNA‐155 negatively affects blood–brain barrier function during neuroinflammation

Discussion

Member takes

Implication

Key Points

Cite This Study

MicroRNA‐155 negatively affects blood–brain barrier function during neuroinflammation

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider