What question did this study set out to answer?

To evaluate the feasibility and efficacy of using TILLING to identify chemically induced mutations in soybean despite challenges posed by its duplicated genome.

January 24, 2008BMC Plant BiologyOpen Access

TILLING to detect induced mutations in soybean

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Authors

JCJennifer L. CooperPresbyterian Hospital BTBradley J. TillUniversity of California, Davis RLRobert G. LaportCollege of Idaho

Discussion

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Implication

Experimental genetic study reveals high induced mutation frequencies in soybean populations, indicating that high-throughput reverse genetics is viable despite extensive genome duplication.

Key Points

To evaluate the feasibility and efficacy of using TILLING to identify chemically induced mutations in soybean despite challenges posed by its duplicated genome.
Applied TILLING to screen seven target genes across four mutagenized soybean populations, comprising three treated with ethyl methanesulfonate (EMS) and one treated with N-nitroso-N-methylurea (NMU).
Pretreated genomic DNA templates with a restriction enzyme prior to PCR amplification to eliminate extraneous targets arising from ancestral genome duplications.
Identified a total of 116 induced mutations across the four populations.
Detected mutation densities of approximately 1/140 kb in the NMU population and one EMS population, approximately 1/250 kb in a second EMS population, and approximately 1/550 kb in the third EMS population.
Restriction enzyme digestion of the template eliminated off-target amplification and facilitated the discovery of four additional mutant alleles.

Cite This Study

Cooper et al. (2008) studied this question.

synapsesocial.com/papers/6a953ac0d33c5efb307cfb61 https://doi.org/10.1186/1471-2229-8-9

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January 24, 2008BMC Plant BiologyOpen Access

TILLING to detect induced mutations in soybean

View Full Paper

Ask AI

Bookmark

Authors

JCJennifer L. CooperPresbyterian Hospital BTBradley J. TillUniversity of California, Davis RLRobert G. LaportCollege of Idaho

Discussion

Member takes

Implication

Experimental genetic study reveals high induced mutation frequencies in soybean populations, indicating that high-throughput reverse genetics is viable despite extensive genome duplication.

Key Points

To evaluate the feasibility and efficacy of using TILLING to identify chemically induced mutations in soybean despite challenges posed by its duplicated genome.
Applied TILLING to screen seven target genes across four mutagenized soybean populations, comprising three treated with ethyl methanesulfonate (EMS) and one treated with N-nitroso-N-methylurea (NMU).
Pretreated genomic DNA templates with a restriction enzyme prior to PCR amplification to eliminate extraneous targets arising from ancestral genome duplications.
Identified a total of 116 induced mutations across the four populations.
Detected mutation densities of approximately 1/140 kb in the NMU population and one EMS population, approximately 1/250 kb in a second EMS population, and approximately 1/550 kb in the third EMS population.
Restriction enzyme digestion of the template eliminated off-target amplification and facilitated the discovery of four additional mutant alleles.

Cite This Study

Cooper et al. (2008) studied this question.

synapsesocial.com/papers/6a953ac0d33c5efb307cfb61 https://doi.org/10.1186/1471-2229-8-9

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