What does this research mean for the field?

Mycobacterium tuberculosis undergoes extensive transcriptional reprogramming of metabolic, respiratory, and stress-response networks during in vivo lung infection, which diverges markedly from standard in vitro culture profiles. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Characterize the genome-wide transcriptional landscape of Mycobacterium tuberculosis directly within diseased host lungs to determine in vivo adaptation and survival mechanisms.

August 31, 2026Frontiers in ImmunologyOpen Access

Genome-wide transcriptional landscape of Mycobacterium tuberculosis during acute lung infection

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Authors

GBGunapati Bhargavi SGSaleena Ghanny PSPatricia Soteropoulous

Discussion

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Overview

In vivo transcriptomic profiling reveals extensive metabolic and stress reprogramming in rabbit lungs with active tuberculosis, highlighting distinct survival pathways for drug targeting.

Key Points

Characterize the genome-wide transcriptional landscape of Mycobacterium tuberculosis directly within diseased host lungs to determine in vivo adaptation and survival mechanisms.
Conducted microarray-based genome-wide transcriptomic profiling of Mycobacterium tuberculosis (Mtb) using RNA extracted directly from infected rabbit lung homogenates.
Compared in vivo bacterial gene expression against standard in vitro axenic broth-culture controls to identify differentially regulated pathways across microenvironments.
Mtb demonstrated extensive reprogramming of metabolic, respiratory, and stress-response networks in vivo that markedly diverged from broth-culture expression patterns.
In vivo infection selectively upregulated pathways responsible for stress adaptation, lipid catabolism, nucleic acid metabolism, and transcriptional regulation inside granulomatous lesions.

Cite This Study

Bhargavi et al. (2026) studied this question.

synapsesocial.com/papers/6a9541eaf20e493292a7421d https://doi.org/10.3389/fimmu.2026.1875227

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August 31, 2026Frontiers in ImmunologyOpen Access

Genome-wide transcriptional landscape of Mycobacterium tuberculosis during acute lung infection

View Full Paper

Ask AI

Bookmark

Authors

GBGunapati Bhargavi SGSaleena Ghanny PSPatricia Soteropoulous

Discussion

Member takes

Overview

In vivo transcriptomic profiling reveals extensive metabolic and stress reprogramming in rabbit lungs with active tuberculosis, highlighting distinct survival pathways for drug targeting.

Key Points

Characterize the genome-wide transcriptional landscape of Mycobacterium tuberculosis directly within diseased host lungs to determine in vivo adaptation and survival mechanisms.
Conducted microarray-based genome-wide transcriptomic profiling of Mycobacterium tuberculosis (Mtb) using RNA extracted directly from infected rabbit lung homogenates.
Compared in vivo bacterial gene expression against standard in vitro axenic broth-culture controls to identify differentially regulated pathways across microenvironments.
Mtb demonstrated extensive reprogramming of metabolic, respiratory, and stress-response networks in vivo that markedly diverged from broth-culture expression patterns.
In vivo infection selectively upregulated pathways responsible for stress adaptation, lipid catabolism, nucleic acid metabolism, and transcriptional regulation inside granulomatous lesions.

Cite This Study

Bhargavi et al. (2026) studied this question.

synapsesocial.com/papers/6a9541eaf20e493292a7421d https://doi.org/10.3389/fimmu.2026.1875227

View Full Paper

Ask AI

Bookmark