Enantioseparation of antiarrhythmic drugs propafenone and diprafenone, their metabolites and analogs by capillary electrophoresis

The enantioseparation of antiarrhythmic drugs propafenone (PF), diprafenone (DF), their metabolites and analogs was studied by capillary electrophoresis (CE). Several cyclodextrin-type chiral selectors, such as native α, β, and γ-cyclodextrin (CD), selectively and randomly substituted neutral and charged derivatives of β-CD were not suitable for the enantioseparation of PF. The acceptable simultaneous enantioseparation of PF and its major metabolites, 5-hydroxypropafenone (5-OH-PF) and N-despropylpropafenone (N-Des-PF), was achieved with a designed mixture of randomly substituted carboxymethyl-β-CD (CM-β-CD) and sulfated β-CD (SU-β-CD). The method was also developed for the simultaneous enantioseparation of DF and its major metabolites, 5-hydroxydiprafenone (5-OH-DF) and N-desalkyldiprafenone (N-Des-DF) as well as other chiral compounds with a similar structure, such as N-ethylpropafenone and alprafenone.