Complete assignment of the 13C NMR spectrum of vancomycin

The antibiotic vancomycin has been studied by NMR spectroscopy using modern, two-dimensional, 1H–13C correlation techniques. Working with the fully assigned 1H spectrum it has been possible, by a series of experiments designed to detect direct (one-bond) and indirect (multiple-bond)1H–13C couplings, to assign all 66 carbons of the antibiotic. The assignment was carried out in a DMSO solution and the results then translated to an aqueous solution by titration.