What question did this study set out to answer?

To investigate the functional role of UV-C-induced DNA base damage and replication in triggering apoptotic pathways in nucleotide excision repair-deficient cells.

January 1, 2002Molecular Biology of the CellOpen Access

Cell Proliferation and DNA Breaks Are Involved in Ultraviolet Light-induced Apoptosis in Nucleotide Excision Repair-deficient Chinese Hamster Cells

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Authors

TDTorsten DunkernGrünenthal Group (Germany)BKBernd KainaJohannes Gutenberg University Mainz

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Implication

In vitro experiment demonstrates that replication-dependent DNA breaks trigger UV-C-induced apoptosis in repair-deficient fibroblasts, indicating unrepaired lesions require division to cause death.

Key Points

To investigate the functional role of UV-C-induced DNA base damage and replication in triggering apoptotic pathways in nucleotide excision repair-deficient cells.
Compared nucleotide excision repair-deficient Chinese hamster ovary cells mutated in ERCC3 (line 27-1) or ERCC1 (line 43-3B) with repair-proficient control cells (CHO-9 and complemented 43-3B).
Assessed cell proliferation, DNA replication, double-strand break formation, chromosomal aberrations, Bcl-2 expression, and caspase activation following UV-C irradiation and actinomycin D treatment.
Repair-deficient cells displayed pronounced hypersensitivity to UV-C-induced apoptosis, which strictly required active DNA replication and ongoing cell proliferation.
Unrepaired DNA lesions were converted during replication into double-strand breaks and chromosomal aberrations prior to apoptotic onset.
UV-C exposure triggered Bcl-2 downregulation, transcription blockage, and caspase activation independently of functional p53.

Cite This Study

Dunkern et al. (2002) studied this question.

synapsesocial.com/papers/6a954cf9a15fb1352cb2ed36 https://doi.org/10.1091/mbc.01-05-0225

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Implication

In vitro experiment demonstrates that replication-dependent DNA breaks trigger UV-C-induced apoptosis in repair-deficient fibroblasts, indicating unrepaired lesions require division to cause death.

Cell Proliferation and DNA Breaks Are Involved in Ultraviolet Light-induced Apoptosis in Nucleotide Excision Repair-deficient Chinese Hamster Cells

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Implication

Key Points

Cite This Study

Cell Proliferation and DNA Breaks Are Involved in Ultraviolet Light-induced Apoptosis in Nucleotide Excision Repair-deficient Chinese Hamster Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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