Synthesis of Glyco‐Silicas by Cu(I)‐Catalyzed “Click‐Chemistry” and their Applications in Affinity Chromatography

The covalent immobilization of suitable alkyne/azide carbohydrate derivatives on complementarily functionalizated azide/alkyne silica was performed by click ligation througth the Cu(I)‐catalyzed 1,3‐dipolar cycloaddition reaction of such compounds. The new glyco‐silicas have shown to be efficient and valuable bio‐selective affinity chromatographic supports for the purification of lectins as well as for the one‐pot fluorescent labeling of those proteins. The synthetic methodology is simple, high yielding and flexible, allowing the preparation of tailored glyco‐silicas with potential future applications in the inmobilization of other biomolecules.