Automated Procedure for the Estimation of Total Polyphenol Content in Beer, Wort, Malt, and Barley

An automated procedure using 4-aminoantipyrine as a chromogen was used to estimate total polyphenol content in beer, wort, malt, and barley. For 23 beers with a wide range in test results, the correlation between the manual European Brewery Convention method and the automated method was 0.88. Barley and malt determinations were made on 30% aqueous dimethylformamide extracts of grain. Cultivar differences were found, suggesting the use of the procedure to select for polyphenol content in lines from barley breeding programs.