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SUMMARY A strain of staphylococci, chosen for the very marked enhancement by mucin of its intraperitoneal virulence to mice, was cultured in sloppy agar. The supernatant fluids obtained were of low toxicity and were highly potent as active immunizing agents in mice against lethal intraperitoneal challenge by the homologous strain in mucin suspension. The activity of the protective antigen was not affected by boiling for 10 minutes and was retained in the bag during dialysis. The protection could be transferred passively by means of the sera of immunized animals.
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Stephen Fisher (1960) studied this question.
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