T cell cytokine mRNA expression during the course of the immunopathologic ocular disease herpetic stromal keratitis.

Corneal infection with herpes simplex virus in mice induces an inflammatory response in the stroma (herpetic stromal keratitis (HSK)) that appears to represent an immunopathologic reaction in which T cells of the CD4+ subset act as the essential participants. To assess the role of T cell cytokines at different clinical phases of HSK, corneas and draining lymph node (DLN) cells were collected and the levels of mRNA thought to be representative of type 1 and type 2 T cells were quantitated by reverse transcription-PCR. In the corneas collected before the onset of clinical disease, IFN-gamma and IL-4 mRNA were detectable, with levels of IFN-gamma 5- to 15-fold higher than IL-4. During the onset and peak expression of clinical disease, type 1 cytokines IFN-gamma and IL-2 were predominant in the corneas, and IL-4 levels were either very low or undetectable. Neither IL-10 nor IL-5 mRNA was present. After 3 wk postinfection, when some animals with mild disease began to recover, high levels of type 2 cytokine mRNA, particularly IL-10, were present. In addition, only during the recovery phase was IL-10 mRNA present in DLN samples. Levels of transcriptional activity for cytokine mRNA during clinical HSK were higher in corneas than in the corresponding DLN samples. The results indicate that IL-10 may be involved in HSK resolution and that the stimuli for cytokine induction in the cornea may differ from those in the DLN.