What question did this study set out to answer?

Determine the effectiveness of introducing plasmid vector DNA into filamentous fungal protoplasts using high-voltage electroporation.

January 1, 1989Phytopathology

Transformation of Filamentous Fungi with Plasmid DNA by Electroporation

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Authors

MRMark RicheyZhejiang A & F University

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Implication

Laboratory study demonstrates rapid plasmid DNA delivery via short electric pulses in fungal protoplasts, highlighting a chemical-free alternative for hard-to-transform species.

Key Points

Determine the effectiveness of introducing plasmid vector DNA into filamentous fungal protoplasts using high-voltage electroporation.
Exposed fungal protoplasts and plasmid vector DNA to high-amplitude, exponential-decay electric pulses lasting up to 123 ms using a Gene Pulser apparatus.
Evaluated the impact of pulse amplitude and pulse duration on transformation frequency and protoplast viability.
Optimized a rapid, simple transformation procedure that avoids exposure to toxic chemicals.
Provided an alternative transformation route suitable for fungal species that cannot be genetically modified using traditional chemical methods.

Cite This Study

Mark Richey (1989) studied this question.

synapsesocial.com/papers/6a956422d002db7cabf59959 https://doi.org/10.1094/phyto-79-844

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Implication

Laboratory study demonstrates rapid plasmid DNA delivery via short electric pulses in fungal protoplasts, highlighting a chemical-free alternative for hard-to-transform species.

Transformation of Filamentous Fungi with Plasmid DNA by Electroporation

Discussion

Member takes

Implication

Key Points

Cite This Study

Transformation of Filamentous Fungi with Plasmid DNA by Electroporation

Discussion

Member takes

Implication

Key Points

Cite This Study

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